Electrophysiological Study of Sciatic Nerve Regeneration Through Tubes Seeded with Schwann Cells

نویسندگان

  • Hamid Abootaleb Department of Anatomy and Neuroscience Research Center, Iran University of Medical Sciences,
  • Korosh Mansouri Department of Physical Medicine and Rehabilitation, Iran University of Medical Sciences,
  • Mehrdad Bakhtyari Department of Anatomy and Neuroscience Research Center, Iran University of Medical Sciences,
چکیده مقاله:

A B S T R A C TIntroduction: Peripheral nerve injury is a common disorder and leads to permanent neurological defects. Schwann cells have been shown to have nerve repair after being transplanted in peripheral nerve injury. The aim of this study was to determine the beneficial effect of allograft Schwann cells on electrophysiological outcome after transection of the sciatic nerve in rats.Methods: Twenty adult male Wistar rats (200-250 g) were used in this study and left sciatic nerve was cut 10 mm in all of them and randomly divided into two groups. Then, the experimental (n=10) and control (n=10) groups received silicon tube with Schwann cells and silicon tube without Schwann cells respectively. Electrophysiological studies were performed 8 weeks after transplantation. Results: Electrophysiological study in experimental animals showed that amplitude of nerve action potential is higher and latency is less as compared to the control group (p<0.05). Discussion: The combination of different strategies such as silicon tube and Schwann transplantation has a more effective role in nerve repair.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

electrophysiological study of sciatic nerve regeneration through tubes seeded with schwann cells

a b s t r a c tintroduction: peripheral nerve injury is a common disorder and leads to permanent neurological defects. schwann cells have been shown to have nerve repair after being transplanted in peripheral nerve injury. the aim of this study was to determine the beneficial effect of allograft schwann cells on electrophysiological outcome after transection of the sciatic nerve in rats.methods...

متن کامل

Combination of Acellular Nerve Graft and Schwann Cells-Like Cells for Rat Sciatic Nerve Regeneration

OBJECTIVE To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect. METHODS Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twel...

متن کامل

A Silk Fibroin/Collagen Nerve Scaffold Seeded with a Co-Culture of Schwann Cells and Adipose-Derived Stem Cells for Sciatic Nerve Regeneration

As a promising alternative to autologous nerve grafts, tissue-engineered nerve grafts have been extensively studied as a way to bridge peripheral nerve defects and guide nerve regeneration. The main difference between autogenous nerve grafts and tissue-engineered nerve grafts is the regenerative microenvironment formed by the grafts. If an appropriate regenerative microenvironment is provided, ...

متن کامل

Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration

BACKGROUND This study evaluated whether Schwann-like cells (SLCs) induced from bone marrow-derived mesenchymal stem cells (BM-MSCs) transplanted into acellular nerve grafts (ANGs) could repair nerve defects compared with nerve isografts and ANGs with BM-MSCs. METHODS BM-MSCs extracted, separated and purified from the bone marrow of rats, and some of the BM-MSCs were cultured with mixed induct...

متن کامل

Peripheral nerve regeneration through guidance tubes.

Biological nerve grafts have been extensively utilized in the past to repair peripheral nerve injuries. More recently, the use of synthetic guidance tubes in repairing these injuries has gained in popularity. This review focuses on artificial conduits, nerve regeneration through them, and an account of various synthetic materials that comprise these tubes in experimental animal and clinical tri...

متن کامل

Human eyelid adipose tissue-derived Schwann cells promote regeneration of a transected sciatic nerve

Schwann cells (SCs) can promote the regeneration of injured peripheral nerves while the clinical application is limited by donor site complications and the inability to generate an ample amount of cells. In this study, we have isolated human eyelid adipose-derived Schwann cells (hE-SCs) from human eyelid adipose tissue and identified the cell phenotype and function. Using immunofluorescence and...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 1  شماره 3

صفحات  49- 56

تاریخ انتشار 2010-05

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023